Search results for "Deoxyribodipyrimidine Photo-Lyase"

showing 3 items of 3 documents

Molecular and functional analysis of the (6-4) photolyase from the hexactinellid Aphrocallistes vastus.

2003

The hexactinellid sponges (phylum Porifera) represent the phylogenetically oldest metazoans that evolved 570-750 million years ago. At this period exposure to ultraviolet (UV) light exceeded that of today and it may be assumed that this old taxon has developed a specific protection system against UV-caused DNA damage. A cDNA was isolated from the hexactinellid Aphrocallistes vastus which comprises high sequence similarity to genes encoding the protostomian and deuterostomian (6-4) photolyases. Subsequently functional studies were performed. It could be shown that the sponge gene, after transfection into mutated Escherichia coli, causes resistance of the bacteria against UV light. Recombinan…

DNA RepairDNA repairUltraviolet RaysMolecular Sequence DataBiophysicsPyrimidine dimerBiochemistryAnalytical Chemistrychemistry.chemical_compoundComplementary DNAAnimalsAmino Acid SequencePhotolyaseMolecular BiologyGenePhylogenyGeneticsbiologyHexactinellidbiology.organism_classificationRecombinant ProteinsPoriferaSpongechemistryBiochemistryDeoxyribodipyrimidine Photo-LyaseSequence AlignmentDNADNA DamageBiochimica et biophysica acta
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The three-dimensional structure of Drosophila melanogaster (6–4) photolyase at room temperature

2021

A crystal structure of a photolyase at room temperature confirms the structural information obtained from cryogenic crystallography and paves the way for time-resolved studies of the photolyase at an X-ray free-electron laser.

MECHANISMMaterials scienceAbsorption spectroscopyDNA repairfotobiologia02 engineering and technologyCrystal structureREPAIR ACTIVITY03 medical and health sciencesCOLI DNA PHOTOLYASEX-RAY-DIFFRACTIONCryptochromeStructural BiologyAnimalsserial crystallographyCRYSTAL-STRUCTURECRYPTOCHROMEPhotolyaseSERIAL FEMTOSECOND CRYSTALLOGRAPHY030304 developmental biology0303 health sciencesCrystallographyflavoproteinsFADResolution (electron density)TemperaturebanaanikärpänenDNAkidetiede(6-4) photolyase021001 nanoscience & nanotechnologyResearch PapersRADICAL TRANSFER(6–4) photolyaseroom-temperature structureCrystallographyphotolyasesDrosophila melanogasterRECONSTITUTIONX-ray crystallography1182 Biochemistry cell and molecular biologylämpötilaproteiinit0210 nano-technologyDeoxyribodipyrimidine Photo-LyasePHOTOACTIVATIONVisible spectrumActa Crystallographica Section D Structural Biology
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Combined QM/MM investigation on the light-driven electron-induced repair of the (6-4) thymine dimer catalyzed by DNA photolyase.

2013

The (6-4) photolyases are blue-light-activated enzymes that selectively bind to DNA and initiate splitting of mutagenic thymine (6-4) thymine photoproducts (T(6-4)T-PP) via photoinduced electron transfer from flavin adenine dinucleotide anion (FADH(-)) to the lesion triggering repair. In the present work, the repair mechanism after the initial electron transfer and the effect of the protein/DNA environment are investigated theoretically by means of hybrid quantum mechanical/molecular mechanical (QM/MM) simulations using X-ray structure of the enzyme-DNA complex. By comparison of three previously proposed repair mechanisms, we found that the lowest activation free energy is required for the …

Models MolecularDNA RepairLightStereochemistryPyrimidine dimerElectronsPhotochemistryPhotoinduced electron transferQM/MMElectron Transportchemistry.chemical_compoundElectron transferMaterials ChemistryAnimalsPhysical and Theoretical ChemistryPhotolyaseta116ta114Chemistryta1182DNA photolyaseDNASurfaces Coatings and FilmsThymineDrosophila melanogasterPyrimidine DimersBiocatalysisQuantum TheoryProtonsDeoxyribodipyrimidine Photo-LyaseDNAThe journal of physical chemistry. B
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